Researchers have reported a sample-prior-based approach to point spread function decoupling that improves system ...

There is a growing demand for non-invasive insights into the complex three-dimensional subcellular dynamics within living tissues on the frontier of biological research. To achieve simultaneous ...

What is Structured Illumination Microscopy? Structured Illumination Microscopy (SIM) is a super-resolution fluorescence microscopy technique that allows for imaging beyond the diffraction limit of ...

Microscopes have long served as windows into the hidden world of cells, revealing their shape, activity, and chemistry. Yet watching life at this scale has always involved trade-offs. To capture the ...

Researchers have developed a method to determine how reliably target proteins can be labeled using super-resolution fluorescence microscopy. LMU researchers have developed a method to determine how ...

Overcoming the resolution limit in a light microscope of around half a wavelength of light (about 250 nanometers) is one of ...

When single-molecule super-resolution microscopes were first commercialized some 15 years ago, they made headlines for their ability to resolve individual molecules and structures at the nanometer ...

Even those who maintain that super-resolution microscopy is a powerful tool of biological discovery have admitted that it may have a bit of an image problem. For example, in a recent review, several ...

Abiotic stress physiology is a critical field within plant science, particularly as climate change and environmental ...

Computational fluorescence microscopy (CFM) requires accurate point spread function (PSF) characterization for high-quality ...