Learn best practices for sample preparation plus expert tips and tricks for developing an HPLC method.

We will explore reasons for why some methods are quite difficult to transfer to a different HPLC system. In addition, we will look at gradient method development and how to efficiently use a scouting …

1. Theory of liquid chromatography High performance liquid chromatography (HPLC) is a technique for separating analytes dissolved in a liquid, mobile phase by using their specific interaction with a …

Nov 28, 2024 · To optimize HPLC methods, various chromatographic parameters are evaluated, including sample pretreatment, choice of mobile phase, column selection, and detector type. This …

Jun 9, 2025 · In this article, you’ll discover a step-by-step guide to HPLC method development, covering everything from initial planning and separation optimisation to detection, finalisation, and real-world …

Nov 21, 2023 · HPLC is the most commonly used separation technique for detecting, separating, and quantifying drugs. To optimize the method, several chromatographic parameters were investigated, …

Efficient method creation and optimization in HPLC are critical for producing accurate and consistent results while reducing time, cost, and resource requirements. This article explores strategies and …

Jan 29, 2025 · HPLC method development is both an art and a science. By focusing on the fundamentals—like column selection, mobile phase optimization, and detector choice—you can build …

1. Follow preferred method development scheme and do “hands-on” method development – based on selectivity-changing parameters— e g pH column or mobile phase typese.g., pH, column, or mobile …

Sep 20, 2024 · We will discuss about HPLC method development and validation, types of liquid chromatography methods focusing on proper use of a mobile phase like an eluent selection, how to …