pBR322 is a plasmid and was one of the first widely used cloning vectors for E. coli. Created in 1977 in the laboratory of Herbert Boyer at the University of California, San Francisco, it was named after …

pBR322 First-generation E. coli vector for DNA cloning. This plasmid has a low copy number (~20 copies per cell) due to the rop gene.

pBR322 DNA is a common plasmid cloning vector in E. coli that is 4,361bp and contains ampicillin and tetracycline resistance genes.

Aug 8, 2025 · It is a synthetic plasmid and was the first artificial plasmid to be constructed and used as a cloning vector. pBR322 is one of the most studied plasmids. It is 4362 base pairs long. It is …

Plasmid pBR322 is purified from E. coli DH10B. It contains three unique restriction endonuclease recognition sites within the β-lactamase (ampicillin-resistance) gene, eight unique sites within the …

pBR322 is defined as a 4,362-bp double-stranded DNA plasmid cloning vector designed for the preparation of cloned recombinant DNA fragments, containing antibiotic resistance genes, an origin …

The vector pBR322 was constructed in order to have a plasmid with a single PstI site, located in the ampicillin-resistant gene (Apr), in addition to four unique restriction sites, EcoRI, HindIII, BamHI and …

pBR322 is one of the earliest and most widely used cloning vectors in Escherichia coli. Created in 1977 at the University of California, San Francisco, in the laboratory of Herbert Boyer, it is named after …

pBR322 was one of the first plasmids to be completely sequenced, but the sequence has been revised several times. The last revision and review of the sequence information inclicates that two base pairs …

Jun 25, 2025 · In this first article, we will take a look at the commonly used pBR322 plasmid, it’s origin of replication, and the mechanism that controls copy number and replication for pBR322 and related …